Already a short-term soils exposure to the field-rate glufosinate concentration significantly influences soil bacterial communities

To chci

The early impact of glufosinate derived herbicide Basta® 15 on bacterial communities of two different soils never exposed to this herbicide was investigated using cultivation approach and non-cultivation based denaturing gradient gel electrophoresis (DGGE) analysis of amplified 16S rRNA genes. Under the simulated laboratory conditions glufosinate treatment increased numbers of total cultivable heterotrophic bacteria in both tested soils. Surprisingly even the lowest glufosinate concentration (1 mmol) significantly affected bacterial community composition in both tested soils and original populations were replaced by new ones upon the 2 days glufosinate treatment. In nutrient rich Haniska soil the effect was dose dependent and glufosinate treatment decreased genetic diversity of bacterial population. In nutrient poor Kaľava soil the highest glufosinate concentration (16 mmol) increased the diversity of bacterial population probably as a result of carbon source supplementation. Glufosinate treatment selected Gram-negative bacteria in both soils. Two species of Enterobacter genus were found to be dominant in glufosinate treated Haniska soil and Pseudomonas beteli and Brevundimonas diminuta were found to be dominant in glufosinate treated Kaľava soil using non-cultivation based DGGE method. Our data indicated that under the simulated soil conditions the soil bacterial community was significantly affected even by a short-term exposure to glufosinate. … celý popis

Podrobná bibliografie

Hlavní autor

Anna Kopčáková

Další autoři

Jaroslav Legáth, 1957-
Peter Pristaš
Peter Javorský, 1950-

Typ dokumentu

Články

Fyzický popis

4 ilustrace

Publikováno v

Soil and Water Research. -- ISSN 1801-5395. -- Roč. 10, č. 4 (2015), s. 271-277

Témata

méně…

AGRICULTURAL CHEMICALS

PESTICIDES

HERBICIDES

CHEMICAL COMPOSITION

GLUFOSINATE

Pesticide toxicity

PESTICIDAL PROPERTIES

ENZYME INHIBITORS

Impact assessment

SOIL BIOLOGY

SOIL MICROORGANISMS

BIOASSAYS

POPULATION DENSITY

Bacterial counting

PESTICIDE RESISTANCE

BACTERIA

SPECIES

DNA FINGERPRINTING

CHROMOSOME BANDING

PHYLOGENY

agrochemikálie

pesticidy

herbicidy

chemické složení

glufosinate

toxicita pesticidů

působení pesticidů

inhibitory enzymů

posouzení vlivu

půdní biologie

půdní mikroorganizmy

biotesty

hustota populace

počítání baktérií

odolnost k pesticidům

baktérie

druhy

identifikace DNA

proužkování chromozómů

fylogeneze

Košice (Slovensko : oblast)

více… méně…

Popis jednotky

2 fotografie, 2 tabulky

Bibliografie

Seznam literatury na straně 276-277,