Testing chemical agents with the cytokinesis-block micronucleus cytome assay

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In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimetic chemical, indirect-acting spindle poisons and indirect-acting enzyme inhibitors. We looked at the presence of micronuclei as biomarkers for either the loss of chromosome fragments (clastogen) or the loss of a whole chromosome (aneugen), nucleoplasmic bridges as biomarkers for complex rearrangements (e.g., dicentric chromosomes) and nuclear buds as biomarkers for gene amplification. The cytome assay proved to be a suitable tool to investigate genetic effects of environmental agents and to provide insight into their working mechanisms as all chemicals tested showed the expected response. … celý popis

Podrobná bibliografie

Hlavní autor

A. Maes

Další autoři

R. Anthonissen
Luc Verschaeve

Typ dokumentu

Články

Fyzický popis

2 il.

Publikováno v

Folia Biologica. -- ISSN 0015-5500. -- Roč. 58, č. 5 (2012), s. [215]-220

Témata

buňky

genotoxicita

mutageny

inhibitory enzymů

mutace (biologie)

klastogeny

studie

Popis jednotky

1 graf, 1 tb.

Bibliografie

Literatura