Effect of aromatase inhibitor (fadrozole) on proliferation, estradiol production and telomerase activity in pig granulosa cells in vitro

To chci

The objective of the present work was to study the effect of a nonsteroidal aromatase inhibitor (fadrozole) on proliferation, estradiol production, aromatase expression and telomerase activity (TA) in pig granulosa cells (GC) from small (1–2 mm) and large (5–7 mm) follicles. The cells were treated with fadrozole for 48 h and 72 h in basal and FSH-stimulated conditions. Fadrozole caused a decrease of 3H-thymidine incorporation in granulosa cells derived from small (1–2 mm) and large follicles (5–7 mm). The proliferative potential of small-follicle GC was significantly higher under all culture conditions. Estradiol production was suppressed in both granulosa cell populations cultured in the presence of fadrozole for 48 and 72 h. Fadrozole caused a decrease of aromatase gene expression in small-follicle granulosa cell incubated for 72 h and in large-follicle GC after 48 h of culture. Large-follicle GC were characterized by a higher level of estradiol production and aromatase gene expression. Telomerase activity decreased in large-follicle granulosa cells incubated in the presence of an aromatase inhibitor for 72 h. The TA level in large-follicle granulosa cells was higher in comparison to small-follicle GC in all culture conditions after 72 h of incubation. The results of the present study suggest the important role of telomerase in the process of follicular growth and development. … celý popis

Podrobná bibliografie

Hlavní autor

Ewa Chronowska

Další autoři

Milan Tománek
Tomáš Kott

Typ dokumentu

Články

Fyzický popis

4 il.

Publikováno v

Czech Journal of Animal Science. -- ISSN 1212-1819. -- Roč. 54, č. 12 (2009), s. 566-574

Témata

méně…

SOWS

OVARIES

OVARIAN FOLLICLES

DIMENSIONS

GROWTH

CELLS

CULTIVATION

IN VITRO CULTURE

STIMULANTS

PITUITARY HORMONES

GONADOTROPINS

FSH

ENZYME INHIBITORS

TISSUE PROLIFERATION

BIOSYNTHESIS

OESTROGENS

prasnice

vaječníky

vaječníkové folikuly

rozměry

růst

buňky

kultivace

kultura in vitro

stimulanty

hormony hypofýzy

gonadotropiny

inhibitory enzymů

proliferace tkání

biosyntéza

estrogeny

zona granulosa

aromatáza

fadrozol

estradiol

telomeráza

více… méně…

Popis jednotky

4 grafy

Bibliografie

Literatura na s. 573-574