Tartrate-resistant acid phosphatase, matrix metalloproteinase-2 and tissue inhibitor of metalloproteinase-2 in early stages of canine osteoarthritis

To chci

The aim of this study was to determine if the activity of tartrate-resistant acid phosphatase (TRAP) in the synovial fluid (SF) and serum can be used as a marker for diagnosing the early stages of osteoarthritis (OA). We also wished to determine if identifiable differences in the concentrations of matrix metalloproteinase-2 (MMP-2) and tissue inhibitor of matrix metalloproteinase-2 (TIMP-2) could be detected in SF between normal joints and OA joints for the diagnosis of early OA. Ten skeletally mature beagle dogs underwent a unilateral surgical transection of the cranial cruciate ligament and medial meniscectomy. Five sham-operated beagle dogs were used as controls. The synovial fluid was collected in 1, 2 and 3 months and examined by western blotting for MMP-2 and ELISA for TIMP-2. The activity of TRAP in the SF and serum was measured using a spectrophotometer. In addition, the presence of TRAP positive cells in the synovium was identified by enzyme histochemistry. The level of the activity of TRAP and MMP-2 in the SF from the induced OA dogs was significantly higher than that of the control over a three-month period. The TIMP-2 level in the SF was significantly lower in the induced OA dogs than in the control. However, there was no difference in TRAP activity in the serum. Histochemistry revealed a higher number of TRAP positive cells in the synovium from the induced OA dogs. Based on these data, we conclude that the activity of TRAP, MMP-2 and TIMP-2 in SF can be used as a biomarker to diagnose and monitor the early stages of OA. … celý popis

Podrobná bibliografie

Další autoři

H. B. Lee, M. R. Alam, J. W. Seol, Nam-Soo Kim

Typ dokumentu

Články

Fyzický popis

4 il.

Publikováno v

Veterinární medicína. -- ISSN 0375-8427. -- Roč. 53, č. 4 (2008), s. 214-220

Témata

domácí zvířata

psi

choroby zvířat

choroby kloubů

artritida

laboratorní diagnóza

krevní sérum

synoviální mok

kyselá fosfatáza

inhibitory enzymů

PET ANIMALS

DOGS

ANIMAL DISEASES

JOINT DISEASES

ARTHRITIS

LABORATORY DIAGNOSIS

BLOOD SERUM

SYNOVIAL FLUID

ACID PHOSPHATASE

ENZYME INHIBITORS

počáteční stádium

metaloproteináza

Popis jednotky

1 barev. fot., 3 grafy

Bibliografie

Lit. na s. 219-220