Effect of Staphylococcus aureus and Streptococcus uberis on apoptosis of bovine mammary gland neutrophils in vitro

To chci

In the study, 16 mammary glands of four virgin heifers aged 16 to 18 months were examined. Neutrophils were obtained by lavage after an induced influx. After a three-hour incubation of the neutrophils with bacteria Staphylococcus aureus or Streptococcus uberis in vitro, neutrophil apoptosis was detected by morphological features, by determination of histone-associated DNA fragments (ELISA), and by Annexin-V and propidium iodide positivity (flow cytometry). S. aureus and S. uberis reduced the incidence of karyopycnotic and zeiotic neutrophils, and insignificantly reduced the concentration of histone-associated DNA fragments. The incubation of neutrophils with bacteria, however, increased the proportion of Annexin-V-positive cells and Annexin-V and propidium iodide-positive cells. Co-cultivation of neutrophils with either S. aureus or S. uberis led to the induction of phosphatidylserine translocation characteristic of the early stage of apoptosis. The late signs of apoptosis were delayed by co-cultivation of neutrophils with both pathogens. … celý popis

Podrobná bibliografie

Hlavní autor

Zbyšek Sládek

Další autoři

D. Ryšánek
Martin Faldyna

Typ dokumentu

Články

Fyzický popis

1 fot., 4 grafy

Publikováno v

Veterinární medicína. -- ISSN 0375-8427. -- Roč. 50, č. 1 (2005), s. 11-23

Témata

krávy > mléčné žlázy > krevní buňky > leukocyty > granulocyty > apoptóza > Staphylococcus aureus > Streptococcus uberis > pokusy in vitro > kultivační metody > mikroskopie > ELISA > biologická analýza

Popis jednotky

Res. angl

Bibliografie

Lit. s. 21-23 (56 zázn.)